1. Injongo: Le nqubo ihlose ukuhlinzeka ngenqubo emisiwe yokusebenza kwe-aseptic kanye nokuvikelwa kwamagumbi angenalutho.
2. Ububanzi besicelo: ilabhorethri yokuhlola yezinto eziphilayo
3. Umuntu Onomthwalo Wemfanelo: I-QC Supervisor Tester
4.Incazelo: Akukho
5. Izinyathelo zokuphepha
Yenza ngokuqinile imisebenzi ye-aseptic ukuvimbela ukungcoliswa kwamagciwane;opharetha kufanele bacime isibani se-UV ngaphambi kokungena egumbini elingenalutho.
6.Izinqubo
6.1.Igumbi elingenalutho kufanele lifakwe igumbi lokuhlinza eliyinyumba kanye negumbi lokuhlala.Ukuhlanzeka kwegumbi lokusebenza oyinyumba kufanele kufinyelele ekilasini lika-10000. Ukushisa kwangaphakathi kufanele kugcinwe ku-20-24 ° C futhi umswakama kufanele ugcinwe ku-45-60%.Ukuhlanzeka kwebhentshi elihlanzekile kufanele kufinyelele ekilasini le-100.
6.2.Igumbi eliyinyumba kufanele ligcinwe lihlanzekile, futhi kwenqatshelwe ngokuphelele ukunqwabelanisa imfucumfucu ukuze kuvinjelwe ukungcola.
6.3.Vimbela ngokuqinile ukungcoliswa kwazo zonke izinto zokusebenza zokuvala inzalo kanye nemidiya yesiko.Labo abangcolisiwe kufanele bayeke ukuzisebenzisa.
6.4.Igumbi elingenalutho kufanele lifakwe izibulala-magciwane ezisebenzayo, njengesixazululo se-cresol esingu-5%, i-70% yotshwala, isixazululo se-chlormethionine esingu-0.1%, njll.
6.5.Igumbi elingenalutho kufanele lihlanzwe njalo futhi lihlanzwe ngesibulali magciwane esifanele ukuze kuqinisekiswe ukuthi ukuhlanzeka kwegumbi elingenalutho kuyahlangabezana nezidingo.
6.6.Zonke izinto ezisetshenziswayo, izinsimbi, izitsha nezinye izinto ezidinga ukulethwa ekamelweni elingenalutho kufanele zisongwe ziqine futhi zibulawe amagciwane ngezindlela ezifanele.
6.7.Ngaphambi kokungena egunjini elingenalutho, abasebenzi kufanele bageze izandla zabo ngensipho noma ngesibulali magciwane, bese beshintsha izingubo zokusebenza ezikhethekile, izicathulo, izigqoko, imaski namagilavu egumbini le-buffer (noma basule izandla zabo nge-ethanol engu-70%) ngaphambi kokungena ekamelweni elingenalutho.Yenza imisebenzi ekamelweni lebhaktheriya.
6.8.Ngaphambi kokusebenzisa igumbi eliyinyumba, isibani se-ultraviolet egumbini elingenalutho kufanele sivulwe ukuze kukhishwe imisebe nokuvala inzalo imizuzu engaphezu kwengu-30, futhi ibhentshi elihlanzekile kufanele livulwe ngesikhathi esifanayo ukuze livuthe umoya.Ngemuva kokuthi ukuhlinzwa sekuqediwe, igumbi elingenalutho kufanele lihlanzwe ngesikhathi bese lihlanzwa ngokukhanya kwe-ultraviolet imizuzu engu-20.
6.9.Ngaphambi kokuhlolwa, ukupakishwa kwangaphandle kwesampula yokuhlola kufanele kugcinwe kuphelele futhi akufanele kuvulwe ukuze kuvinjelwe ukungcola.Ngaphambi kokuhlolwa, sebenzisa amabhola kakotini angama-70% wotshwala ukuze ubulale amagciwane endaweni engaphandle.
6.10.Phakathi nokusebenza ngakunye, ukulawula okungalungile kufanele kwenziwe ukuze kuhlolwe ukwethembeka kokusebenza kwe-aseptic.
6.11.Lapho umunca uketshezi lwebhaktheriya, kufanele usebenzise ibhola lokumunca ukuze ulumunce.Ungawuthinti utshani ngqo ngomlomo wakho.
6.12.Inaliti yokujova kumele ibulawe inzalo yomlilo ngaphambi nangemva kokusetshenziswa ngakunye.Ngemuva kokupholisa, isiko lingafakwa inoculated.
6.13.Utshani, amashubhu okuhlola, izitsha ze-petri nezinye izitsha eziqukethe uketshezi lwebhaktheriya kufanele zicwiliswe ebhakedeni lokuvala inzalo eliqukethe ingxube ye-Lysol engu-5% yokubulala amagciwane, futhi ikhishwe futhi ihlanjululwe ngemva kwamahora angu-24.
6.14.Uma kunoketshezi lwebhaktheriya oluchithekile etafuleni noma phansi, kufanele uthele ngokushesha isixazululo se-5% ye-carbolic acid noma u-3% we-Lysol endaweni engcolile okungenani imizuzu engu-30 ngaphambi kokuyelapha.Lapho izingubo zokusebenza nezigqoko zingcoliswe uketshezi lwebhaktheriya, kufanele zikhishwe ngokushesha futhi ziwashwe ngemva kokuvala inzalo yesitimu esinomfutho ophezulu.
6.15.Zonke izinto eziqukethe amagciwane aphilayo kufanele zibulawe amagciwane ngaphambi kokuba zihashazwe ngaphansi kompompi.Akuvunyelwe ngokuphelele ukungcolisa indle.
6.16.Inani lamakholoni ekamelweni eliyinyumba kufanele lihlolwe nyanga zonke.Uma ibhentshi elihlanzekile livuliwe, thatha izitsha eziningi ze-petri eziyinyumba ezinobubanzi bangaphakathi obungama-90 mm, bese ujova nge-aseptically cishe u-15 ml we-agar culture medium encibilikisiwe futhi yapholiswa yaba ngu-45°C.Ngemva kokuqiniswa, yibeke ibheke phansi ku-30 kuya ku-35 Fudumeza amahora angu-48 ku-incubator engu-℃.Ngemuva kokukhombisa ubunyumba, thatha amapuleti ama-3 kuye kwayi-5 uwabeke kwesokunxele, maphakathi nakwesokudla sendawo yokusebenza.Ngemva kokuvula ikhava futhi uwaveze imizuzu engu-30, uwabeke ubheke phansi ku-incubator engu-30 kuya ku-35°C amahora angu-48 futhi uwakhiphe.hlola.Isilinganiso senani lamabhaktheriya anhlobonhlobo epuletini endaweni ehlanzekile yekilasi le-100 akumele lidlule ikoloni elingu-1, futhi inombolo emaphakathi egumbini elihlanzekile lekilasi elingu-10000 akufanele lidlule amakoloni angu-3.Uma umkhawulo weqiwe, igumbi elingenalutho kufanele libulawe amagciwane kuze kube yilapho ukuhlolwa okuphindaphindiwe kuhlangabezana nezidingo.
7. Bheka isahluko (Indlela Yokuhlola Inzalo) kokuthi "Izindlela Zokuhlola Izidakamizwa Ezihlanzekile" kanye "Nemikhuba Ejwayelekile Yokusebenza YaseShayina Yokuhlola Izidakamizwa".
8. Umnyango Wokusabalalisa: Umnyango Wokuphatha Ikhwalithi
Isiqondiso sezobuchwepheshe segumbi elihlanzekile:
Ngemva kokuthola indawo eyinyumba nezinto eziyinyumba, kufanele sigcine isimo esiyinyumba ukuze sifunde i-microorganism ethile eyaziwayo noma sisebenzise imisebenzi yayo.Uma kungenjalo, ama-microorganisms ahlukahlukene avela ngaphandle angahlangana kalula. Isenzakalo sokuxutshwa kwama-microorganisms angabalulekile avela ngaphandle kubizwa ngokuthi amagciwane angcolisayo ku-microbiology.Ukuvimbela ukungcola kuyindlela ebalulekile emsebenzini we-microbiological.Ukuvala inzalo okuphelele ngakolunye uhlangothi kanye nokuvimbela ukungcola ngakolunye kuyizici ezimbili zendlela ye-aseptic.Ngaphezu kwalokho, kufanele sivimbele ama-microorganisms esicwaningwayo, ikakhulukazi ama-microorganisms e-pathogenic noma ama-microorganisms enziwe ngofuzo angekho ngokwemvelo, ekuphunyukeni ezitsheni zethu zokuhlola aye endaweni yangaphandle.Ngalezi zinhloso, ku-microbiology, kunezinyathelo eziningi.
Igumbi eliyinyumba ngokuvamile liyigumbi elincane elakhiwe ngokukhethekile elabhorethri ye-microbiology.Ingakhiwa ngamashidi nengilazi.Indawo akufanele ibe nkulu kakhulu, cishe amamitha-skwele angu-4-5, futhi ukuphakama kufanele kube ngamamitha angu-2.5.Igumbi le-buffer kufanele limiswe ngaphandle kwegumbi eliyinyumba.Umnyango wegumbi lebhafa kanye nomnyango wegumbi eliyinyumba akufanele ubhekane nendawo efanayo ukuze uvimbele ukugeleza komoya ekuletheni amagciwane ahlukahlukene.Kokubili igumbi elingenalutho kanye negumbi lebhafa kumele lingangeni moya.Imishini yokufaka umoya yasendlini kufanele ibe nemishini yokuhlunga umoya.Iphansi kanye nezindonga zegumbi eliyinyumba kufanele kube bushelelezi, kube nzima ukugcina ukungcola futhi kube lula ukuhlanza.Indawo yokusebenza kufanele ibe sezingeni.Kokubili igumbi eliyinyumba negumbi lebhafa kufakwe izibani ze-ultraviolet.Amalambu e-ultraviolet egumbini eliyinyumba aqhele ngemitha elingu-1 ukusuka endaweni yokusebenza.Abasebenzi abangena egumbini elingenalutho kufanele bagqoke izingubo nezigqoko ezingenazinza.
Njengamanje, amagumbi angenalutho atholakala kakhulu ezimbonini ze-microbiology, kuyilapho amalabhorethri avamile asebenzisa ibhentshi elihlanzekile.Umsebenzi oyinhloko webhentshi elihlanzekile ukusebenzisa idivayisi ye-laminar yokugeleza komoya ukususa uthuli oluhlukahlukene oluhlanganisa ama-microorganisms endaweni yokusebenza.Idivayisi kagesi ivumela umoya ukuthi udlule kusihlungi se-hepa bese ungena endaweni yokusebenza, ukuze indawo yokusebenza ihlale igcinwe ngaphansi kokulawulwa komoya oyinyumba ogelezayo.Ngaphezu kwalokho, kunekhethini lomoya elinesivinini esiphezulu ohlangothini oluseduze nangaphandle ukuvimbela umoya we-bacteria wangaphandle ukuthi ungangeni.
Ezindaweni ezinezimo ezinzima, amabhokisi okhuni angenalutho angasetshenziswa esikhundleni sebhentshi elihlanzekile.Ibhokisi eliyinyumba linesakhiwo esilula futhi kulula ukuhamba.Kunezimbobo ezimbili ngaphambili kwebhokisi, ezivinjwe izicabha zokudonsa uma zingasebenzi.Ungakwazi ukunweba izingalo zakho phakathi ngesikhathi sokusebenza.Ingxenye engenhla yangaphambili ifakwe ingilazi ukuze kube lula ukusebenza kwangaphakathi.Kukhona isibani se-ultraviolet ngaphakathi kwebhokisi, futhi izitsha namagciwane angafakwa ngomnyango omncane ohlangothini.
Amasu okusebenza e-Aseptic okwamanje awadlali indima ebalulekile kuphela ocwaningweni lwe-microbiological kanye nezinhlelo zokusebenza, kodwa futhi asetshenziswa kabanzi kubuchwepheshe bezinto eziphilayo.Isibonelo, ubuchwepheshe be-transgenic, ubuchwepheshe be-monoclonal antibody, njll.
Isikhathi sokuthumela: Mar-06-2024