1. Inhloso: Le nqubo ihlose ukuhlinzeka ngenqubo evamile yokusebenza kwe-aseptic nokuvikelwa kwamakamelo ayinyumba.
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I-3. Umuntu onesibopho: I-QC Supervisor Tenser
4..efinition: akekho
I-5. Izindlela zokuphepha zokuphepha
Enza ngokuqinile imisebenzi ye-aseptic ukuvikela ukungcoliswa kwe-microbial; Ama-opharetha kufanele acime isibani se-UV ngaphambi kokungena egumbini elinyumba.
I-6.procerures
6.1. Igumbi eliyinyumba kufanele lihlome ngegumbi lokusebenza oyinyumba kanye negumbi le-buffer. Ukuhlanzeka kwegumbi lokusebenza oyinyumba kufanele kufinyelele ekilasini 10000. Izinga lokushisa lasendlini kufanele ligcinwe ku-20-24 ° C kanti umswakama kufanele agcinwe ku-45-60%. Ukuhlanzeka kwebhentshi elihlanzekile kufanele kufinyelele ekilasini 100.
6.2. Igumbi eliyinyumba kufanele ligcinwe lihlanzekile, futhi linqatshelwe ngokuphelele ukugcizelela imfucumfucu ukuvikela ukungcoliswa.
6.3. Vimbela ngokuqinile ukungcoliswa kwayo yonke imishini yokufaka inzalo kanye nemidiya yesiko. Labo abangcoliswe kufanele bayeke ukusebenzisa bona.
6.4. Igumbi eliyinyumba kufanele lifakwe ama-disninfectants asebenzayo, njenge-5% cresol solution, utshwala abangama-70%, 0.1% chlormethionione solution, njll.
6.5. Igumbi eliyinyumba kufanele licwebezelwe njalo futhi lihlanzwa ngokubulala amagciwane afanele ukuze kuqinisekiswe ukuthi ukuhlanzeka kwegumbi eliyinyumba kuhlangabezana nezidingo.
6.6. Zonke izinsimbi, izinsimbi, izitsha nezinye izinto ezidinga ukulethwa egumbini elinyumba kufanele zisongwe ngokuqinile futhi zicwengeke ngezindlela ezifanele.
6.7. Ngaphambi kokungena egumbini elinyumba, abasebenzi kumele bageze izandla zabo ngensipho noma bebulala amagciwane, bese beshintsha babe izingubo zomsebenzi ezikhethekile, izicathulo, izigqoko, imaski kanye namagilavu e-70%) ngaphambi kokungena egumbini elinyulu. Yenza imisebenzi ku-bacterial Chamber.
6.8. Ngaphambi kokusebenzisa igumbi elinyumba, isibani se-ultraviolet egumbini elinyumba kufanele sivulwe ukuze i-irradiation kanye nokubulawa inzalo isikhathi esingaphezu kwemizuzu engama-30, futhi ibhentshi elihlanzekile kufanele livulwe ukuze lishaye umoya ngasikhathi sinye. Ngemuva kokuthi umsebenzi usuqediwe, igumbi elinyumba kufanele ahlanzwe ngesikhathi bese licwengeka kancane nge-ultraviolet light imizuzu engama-20.
6.9. Ngaphambi kokuhlola, ukufakwa kwangaphandle kwesampula yokuhlola kufanele kugcinwe kungaqinile futhi akumele kuvulwe ukuvikela ukungcoliswa. Ngaphambi kokuhlola, sebenzisa amabhola ekotini angu-70% ukuze abulale amagciwane ngaphezulu.
6.10. Ngesikhathi sokuhlinzwa ngakunye, kufanele kwenziwe ukulawulwa okungalungile ukubheka ukwethenjwa kokusebenza kwe-aseleic.
6.11. Lapho uthatha uketshezi lwama-bacteriya, kufanele usebenzise ibhola lokumunca ukuze limukele. Ungathinti utshani ngqo ngomlomo wakho.
6.12. Inaliti ye-inoculation kumele ihlanzwe yilangabi ngaphambi nangemva kokusetshenziswa ngakunye. Ngemuva kokupholisa, amasiko angenziwa.
6.13. Amashubhu, amashubhu wokuhlola, izitsha ze-petri kanye nezinye izinto eziqukethe uketshezi lwamagciwane kufanele zifakwe kubhakede lokufaka inzalo aqukethe isixazululo se-magciwane esingu-5% ukuze zibulawe amagciwane, futhi zikhishwe futhi zihlanjululwe ngemuva kwamahora angama-24.
6.14. Uma kukhona uketshezi lwamagciwane oluchithwe etafuleni noma phansi, kufanele uthele ngokushesha 5% i-carbolic acid solution noma i-3% Lysol endaweni engcolisiwe okungenani imizuzu engama-30 ngaphambi kokuyiphatha. Lapho izingubo nezigqoko zomsebenzi zingcoliswe ngoketshezi lwegciwane, kufanele zisuswe ngokushesha futhi zihlanzwa ngemuva kokubulawa inzalo okuthe xaxa.
6.15. Zonke izinto eziqukethe ama-bacterium bukhoma kufanele zibulawe amagciwane ngaphambi kokuba zihlatshwe ngaphansi kompompi. Kuvunyelwe ngokuphelele ukungcolisa indle.
6.16. Inani lamakholoni egumbini elinyumba kufanele lihlolwe njalo ngenyanga. Ngebhentshi elihlanzekile livulekile, thatha inombolo yezitsha ze-petri ezingenamatheli ezinobubanzi obungu-90 mm, bese ujova cishe nge-15 ml ye-agar yesiko le-agar cute ancibilikile futhi apholile cishe ngo-45 ° C. Ngemuva kokuqina, yibeke phansi phansi ngo-30 kuye ku-35 incubate amahora angama-48 ku-ancubator ℃. Ngemuva kokufakazela inzalo, thatha amapuleti amathathu kuya kwayi-5 bese uwabeka ngakwesobunxele, maphakathi nakwesokudla kwesikhundla. Ngemuva kokuvula isembozo futhi uziveze imizuzu engama-30, zibeke zibheke phansi ku-30 kuye ku-35 ° C incubator amahora angama-48 bese zizikhipha. hlola. Inani elijwayelekile lamagciwane ahlukile epuletini endaweni ehlanzekile ye-Class 100 ngeke adlule ikoloni eli-1, bese kuthi inombolo ephakathi egumbini elihlanzekile le-10000 ngeke lidlule amakoloni ama-3. Uma umkhawulo udluliwe, igumbi eliyinyumba kufanele libulawe amagciwane kuze kube yilapho kuhlolwe okuphindaphindiwe kuhlangabezana nezidingo.
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8. UMnyango Wezokusabalalisa: UMnyango Wokulawulwa Kwekhwalithi
Ukuhlanza Isiqondisi Sezobuchwepheshe Esihlanzekile:
Ngemuva kokuthola imvelo oyinyumba nezinto ezinyumba, kufanele silondoloze isimo esiyinyumba ukuze sifunde ama-microorganism owaziwayo noma asebenzise imisebenzi yawo. Ngaphandle kwalokho, ama-microorganisms ahlukahlukene avela ngaphandle angaxubana kalula. Into yokuxubana yokuxuba ama-microorganisms kusuka ngaphandle ibizwa nge-contamioting. Ukuvimbela ukungcoliswa kuyindlela ebalulekile emsebenzini we-microbiological. Qedela inzalo ngakolunye uhlangothi nokuvimbela ukungcoliswa kwezinye kuyizinto ezimbili zenqubo ye-aseptic. Ngaphezu kwalokho, kufanele sivimbele ama-microorganisms ngaphansi kokutadisha, ikakhulukazi ama-microgenic microorganisms noma ama-microorganisms anezinkinga zofuzo ezingekho emvelweni, kusukela ekuphunyukeni ezitsheni zethu zokuhlola emvelweni yangaphandle. Ngalezi zinhloso, nge-microbiology, kunezindlela eziningi.
Igumbi eliyinyumba livame ukusetha igumbi elincane ngokukhethekile efebhuri ye-microbiology. Ingakhiwa ngamashidi nengilazi. Le ndawo akufanele ibe nkulu kakhulu, cishe amamitha-skwele angama-4-5, futhi ukuphakama kufanele kube ngamamitha ayi-2,5. Igumbi elibufebe kufanele lisethelwe ngaphandle kwegumbi eliyinyumba. Umnyango wegumbi le-buffer nomnyango wegumbi eliyinyumba akufanele babhekane nendlela efanayo ukuvikela ukuhamba kwendiza ekuletheni amagciwane amaxinda. Zombili igumbi elinyumba negumbi le-buffer kumele zibe nomoya. Okokusebenza komoya omncane kufanele kube namadivayisi we-Filtration asolwandle. Phansi nezindonga zegumbi elinyumba kufanele kube bushelelezi, kunzima ukungcoliswa ukungcola futhi kube lula ukuwahlanza. Indawo yomsebenzi kufanele ibe luveli. Kokubili igumbi elinyumba kanye negumbi le-buffer lihlome ngamalambu e-ultraviolet. Izibani ze-ultraviolet egumbini elinyumba ziyimitha eyi-1 kude nomsebenzi. Abasebenzi abangena egumbini elinyumba kufanele bagqoke izingubo nezigqoko ezingcolile.
Njengamanje, amakamelo ayinyumba iningi akhona ezindaweni ezinobuchwepheshe be-microbiology, kanti ama-lateratories ajwayelekile asebenzisa ibhentshi elihlanzekile. Umsebenzi oyinhloko webhentshi elihlanzekile ukusebenzisa i-Laminanar Air Flow divayisi ukususa amathuli amancane amancane kufaka phakathi ama-minroorganisms asemsebenzini. Idivaysi kagesi ivumela umoya ukuthi udlule kuhlu lwe-hepa bese ufaka indawo yomsebenzi, ukuze indawo yomsebenzi igcinwe njalo ngaphansi kokulawulwa komoya oyinyumba ogelezayo. Ngaphezu kwalokho, kunesivinini somoya esisheshayo esiseceleni ohlangothini olusondele ngaphandle ukuvikela umoya we-bacterial wangaphandle ongangenela.
Ezindaweni ezinemibandela enzima, amabhokisi oyinyumba okhuni nawo angasetshenziswa esikhundleni sebhentshi elihlanzekile. Ibhokisi elinyumba linesakhiwo esilula futhi kulula ukuhambisa. Kunezimbobo ezimbili ezingaphambi kwebhokisi, ezivinjelwe yi-Push-download Doors uma zingasebenzi. Unganweba izingalo zakho ngesikhathi sokusebenza. Ingxenye engenhla yomphambili ifakwe ingilazi ukwenza lula ukusebenza kwangaphakathi. Kukhona isibani se-ultraviolet lang Inside ebhokisini, futhi izitsha namagciwane kungafakwa ngokusebenzisa umnyango omncane ohlangothini.
Amasu wokusebenza we-Aseptic njengamanje awadlali nje kuphela iqhaza elibalulekile ekucwaningweni kwe-microbiologiological kanye nezicelo, kodwa futhi asetshenziswa kabanzi kuma-biotechnologies amaningi. Isibonelo, ubuchwepheshe be-transgenic, ubuchwepheshe be-monoclonal antibody technology, njll.
Isikhathi sePosi: Mar-06-2024